Kurotchenko S.P., Subbotina T.I., Tuktamyshev I.I., Tuktamyshev I.Sh, Khadartsev A.A., Yashin A.A.

State Unitary Enterprise, Institute of New Medical Technologies, Tula, Russia.

Shielding effect of mineral Carbon60 during electromagnetic irradiation of rats


A team of researchers wanted to study the effect of electromagnetic radiations on Wistar rats that were shielded with Carbon60. Carbon60 has been used for more than 300 years to treat various diseases. There has been increased interest in this mineral as more and more scientists are using it for research purposes. The current technological world is filled with electromagnetic radiations that are harmful to human health.


Here they studied the effect of low intensity microwave 1 GHz electromagnetic radiation on the blood cells and bone marrow of the shielded rats. The microwave radiations were biotropic and were not hazardous. 1 GHz EMR causes pathological processes affecting the vegetative nervous system, reproductive system, and blood formation system.

Particular emphasis was given on the ability of Carbon60 to reduce the effect of radiations under these circumstances. The research was conducted to establish whether Carbon60 can shield the rats from radiations.

Materials and methods

The researchers studied the shielding effect of Carbon60 using two groups of Wistar rats: control and experimental. Control and experimental rats were exposed to equal radiations. The frequency of the radiations was 1 GHz. One EMR exposure lasted 30 minutes; total exposure time was three hours. The characteristics of the red bone marrow and the blood cells were examined for 24, 48 and 72 hours respectively as well as on the sixth day of the experiment. Since the pathological alterations depend on the period of exposure to these radiations, they estimated the indexes after every 15 minutes.


They evaluated the morphological properties of red bone marrow cells, count of erythrocytes, the concentration of hemoglobin and leukocytes count. Samples of blood were taken from the caudal vein of the rat. The blood smears were stained using Romanovsky-Giemsa method. The count of the red blood cells were estimated using a S_4 stimul Plyus Laboratory counter through the modified method. Before the experiment the blood indexes were corresponding to normal. 

The researchers classified the rats into two groups. Both groups were exposed to microwave radiations. After exposing the two groups to these radiations they investigated the total leukocyte count in each group. They were exposed to radiations where they were rotated right-handed or left-handed to the radiation using a rotating plane. The polarization plane was either right-handed (D-form, D-EMR) or left –handed ( L-form, L-EMR).

The total leukocyte count in the first group had decreased after 72 hours of exposure to L-EMR. Hemoglobin content, erythrocyte count had also significantly reduced after exposure. The pathological alterations in the first blood smear included nuclei hyper segmentation and hypertrophy.

The second group was exposed to D-EMR for seventy two hours showed a considerable reduction of leukocytes. The morphological changes found in here included the occurrence of neutrophils that had hyper segmented nuclei in their blood smears. 


The development of morphological and functional reactions in the system of blood formation and peripheral blood is slower when Carbon60 is used for shielding. Carbon60, as a shielding factor, suppresses and slows down the development of pathological reactions in response to the exposure to 1 GHz  EMR. Changes in hematological values in the control and experimental group when exposed to 1 GHz EMR are similar to those when exposed to 37 GHz EHF EMR which indicates a common damaging mechanism. It should be understood that at later stages L-EMR showed a decrease in leukocyte count. But exposure to D_EMR had no effect on the blood cell count.


From their results, it is clear that Carbon60 shielded the rats from the damaging effect of microwave radiations. This proves that Carbon60 has positive shielding effect from electromagnetic radiations.


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The health effect of EMR with a range of 1 GHz was studied on human volunteers

The volunteers demonstrated severe bradycardia, increased electrokinetic energy of the buccal epithelium nucleuses, changes in the brain action currents during the initial period of EMR exposure; decrease in cerebral circulation and blood pressure; signs of anxiety, insignificant changes in 〈- and ®-rhythm in their EEG; suppression of slow waves in their EEG in the central, temporal and occipital regions, which means that EMR can produce effects on the brain action currents (however, its effect on the vital activity is still unclear). Out of six adenohypophysis hormones for thyrotropine a 21 % decrease in concentration in plasma was revealed. Changes in the function of the CNS and cardiovascular system were seen; head skin temperature increased by 4.7 оС and eardrum temperature by more than 0.5 оС. Insignificant changes in sleep, response to alcohol, memory, and cognitive capabilities were seen.

An increase in the range of brain action currents was the most significant after EMR was shut off for 15 to 20 minutes, meaning it is the effect of EMR on human health. Transient changes in the brain bioelectrical activity and hormonal activity shifts were observed. Local thermal effect of cellular communications can be reduced by means of Carbon60 shielding. Data on ten human volunteers exposed to EMR once for 10 minutes are provided in the table below.

Comparative temperature changes before and after ERM exposure

EMR with various carrier frequencies, modulation frequencies and emitted by cell phones has effects on the brain and peripheral reception areas of the vestibular and auditory analyzer and retina with varying depth distribution and different doses absorbed.

Experimental research of the effect of EMR at a range of 1 GHz on humans and animals

Below are the results of the experiments which we consider to be the control ones with regard to the previous experiments involving Carbon60 shielding.

Experiments were conducted on C57BL/6 mice (to easily register possible mutagenic effects) and random breed mice. The experiment was conducted on groups of mice, each including two families: C57BL/6 mice and random breed mice. The families were simultaneously exposed to EMR for 15 minutes once a week for one month. During the first exposure, immediately after the apparatus was turned on, the experimental mice displayed abnormal behavior. When exposed to EMR, the mice began rushing about the cage, showed aggression to one another, and refused to eat. Their excitement lasted for 3 to 5 minutes after which the mice demonstrated passive behavior with signs of fear. Specifically, they shrank into the corners of their cage, bit, and did not let people touch them. Excitement and aggression were seen in the course of the whole experiment. The random breed mice displayed significantly more excitement and aggression, while some of the C57BL/6 mice proved EMR resistant and their behavior did not change. Upon the completion of the experiment, persistent behavioral abnormalities were seen in one family of C57BL/6 mice and two families of random breed mice. In these two families the male mouse displayed aggression to the female mice, and in one family of C57BL/6 mice one of the female mice displayed aggression to the male mouse. The families with abnormal behaviors had to be withdrawn from the experiment. Baby mice were born in six families (three C57BL/6 and three random breed). All baby mice were born healthy and with no visible abnormalities. In order to identify the effect of EMR on newborn mice, two female mice with 2-day-old baby mice were introduced into the experiment. When exposed to EMR, the random breed female mouse became highly aggressive biting five baby mice to death. The C57BL/6 female mouse remained calm and displayed no aggression to her baby mice. In two days after being exposed to EMR, 3 out of 6 baby mice survived: 1 male baby mouse and 2 female baby mice which were not involved in the experiment as a new family. After the third exposure, two adult random breed female mice died within 3 to 3.5 hours after being exposed to EMR. Autopsy revealed that the mice had developed ascites and mesenteric hyperemia, and had enlarged liver and spleen. Macroscopic changes in the organs of the dead mice indicate severe microcirculatory disorders and development of portal hypertension. Results of the experiments conducted on families consisting of the mice which had survived were similar to those of the first set of experiments. The male mouse in the family of C57BL/6 mice displayed aggression and had to be withdrawn from the experiment. No baby mice were born in the family of random breed mice after EMR exposure despite their normalized behavior. Four families of C57BL/6 mice and three families of random breed mice showed no response to ERM in the course of the experiment. This indicates the pathogenic effect of ERM on human health involving damaging both central and peripheral mechanisms of homeostatic regulation as well as the importance of individual EMR resistance. The lack of mutagenic effect is doubtful because it is very difficult to reveal mutations in a limited number of generations. A discharge oscillator was used meaning the ERM spectrum included other frequencies, however most of them were within the 450–1,800 MHz range for cellular communications.

The body of an animal is exposed to EMR. However, it appears more reasonable to have the animal’s head exposed to EMR for people using cellphones generally have their heads exposed to EMR.



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